Lymphoid and myeloid defects in Pofut1-null mice were caused by both cell-autonomous mechanism(s) and environmental cues. A total of 2 million donor bone marrow cells isolated from either WT (Ly5.2) or pIpC-treated Mx-Cre/Pofut^F/F (Ly 5.2) were delivered via tail vein to lethally irradiated (9.5 Gy) WT (Ly5.1) or Mx-Cre/Pofut1^F/F (Mx-Cre/P^F/F) recipients. (A) Peripheral neutrophil numbers were enumerated 3 months after transplantation. (B) FACS analysis of recipient marrow myeloid progenitors derived from WT or Mx-Cre/P^F/F marrow cells in WT or Mx-Cre/P^F/F recipients by gating on the Ly5.2⁺ cells. Populations of CMP or GMP are shown as percentages of Lin⁻Sca-1⁻IL-7R⁻c-kit⁺ cells. (C-E) FACS analysis of recipient spleens for Gr-1 expression (C), CD23 versus CD21 expression on B220⁺ cells (D), and plots of absolute numbers of splenic MZB cells (E). (F) Enumeration of total thymocytes derived from donors in recipients 3 months after transplantation. (G-H) FACS analysis of donor-derived thymocyte CD8 versus CD4 expression (G) and DN1-DN4 development defined by expression of CD44 and CD25 on CD4⁻CD8⁻ (DN) cells (H). Percentages of CD44⁺CD25⁻ (DN1), CD44⁺CD25⁺ (DN2), CD44⁻CD25⁺ (DN3), and CD44⁻CD25⁻ (DN4) are indicated in the quadrants. (B-D,G-H) Data are representative of 3 independent experiments with 1 to 3 mice of each donor-recipient pair per experiment. (A,F) Histograms represent the mean ± SD of 4 to 6 mice for each donor-recipient pair. Student t test was performed to compare the numbers (A,E-F) among different donor-recipient pairs. P values that are significantly different are shown as indicated.