Myeloid hyperplasia and reduced lymphopoiesis in Mx-Cre/Pofut1^F/F mice. (A) PB neutrophil counts in control mice (Mx-Cre/Pofut1^F/+ or Pofut1^F/F) or Mx-Cre/Pofut1^F/F mice 3 to 5 months after pIpC injection. Data are mean ± SD of 3 independent experiments with 2 or 3 mice of each genotype per experiment. Statistical analysis was by the Student t test. (B-C) FACS analysis of the percentage of granulocytes (Gr-1⁺), B lymphocytes (B220⁺), and T lymphocytes (CD4⁺/CD8⁺) in the periphery (B) and bone marrow (C). (D) FACS analysis of bone marrow myeloid progenitors (CMP: Lin⁻c-kit⁺Sca-1⁻IL-7R⁻CD34⁺FcγRII^low; GMP: Lin⁻c-kit⁺Sca-1⁻IL-7R⁻CD34⁺FcγRII⁺; megakaryocyte-erythroid progenitor: Lin⁻c-kit⁺Sca-1⁻IL-7R⁻CD34^lowFcγRII^low). Populations of CMP or GMP are shown as percentages of Lin⁻Sca-1⁻IL-7R⁻c-kit⁺ cells. (E) Representative gross anatomy and hematoxylin and eosin staining of pIpC-treated control or Mx-Cre/Pofut1^F/F mice spleen. The hematoxylin and eosin slides of mice were photographed using Olympus BX41 microscope (numeric aperture of the objective lens 20×/0.50) and the Spot software Version 4.7 (Diagnostic Instruments Inc). (F) FACS analysis of granulocytes, B and T lymphocytes in spleens from pIpC-treated control or Mx-Cre/Pofut1^F/F mice. (B-F) Data are representative of at least 4 independent experiments with 2 mice of each genotype per experiment.