ΔHmga2 expression leads to increased EPO-independent phophorylation of AKT. (A) Validation of microarray analysis using qRT-PCR showed similar increase in the expression of Jak2 mRNA in KLS cells from ΔHmga2 mice compared with WT mice. Representative results of ddCT assay using same samples as used in the microarray analysis are shown. (B) Flow cytometry of pStat3 (left) and pStat5 (right) in KLS cells in lineage-depleted BM cells. (C) Western blots of total BM cells incubated in the absence or presence of EPO and IL3. Each protein was examined using a single gel. Vertical lines between WT and ΔHmga2 have been inserted to indicate repositioned gel lanes in JAK2 and pStat5. Data are representative of triplicate experiments in flow cytometry and Western blots. (D) Band intensities of phosphorylated proteins in Western blots measured using infra red imaging (Odyssey). *P < .05.