CB1 antagonism inhibits FAK, JNK, Rho, and MMP-2 activation. Endothelial cells were pretreated with CB1 antagonist (30 minutes, 0.3μM) and stimulated with bFGF (10 ng/mL) for 24 hours. (A) Anti-FAK immunoprecipitates (IPs) were analyzed with anti-phosphotyrosine antibody. (B) Western blot analysis of extracts using phospho-JNK (Thr183/Tyr185) antibody. (C) Rho activity (level of GTP-bound Rho) was detected by Western blotting. (A-C) Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD; ANOVA vs control, **P < .01, ***P < .001). (D) MMP-2 activity was detected by gelatin zymography. HUVECs stimulated with PMA (0.1mM, 24 hours) were used as positive control for MMP-2 activity. The relative pixel density for the 72 kDa MMP-2 is shown. Data are presented as mean ± SE of 3 independent experiments (ANOVA, ***P < .001 vs control).