Deficiency in PML impairs IL-1β secretion in macrophages. (A-C) Reduced IL-1β secretion in PML-null macrophages. Bone marrow–derived macrophages (BMDMs) from normal littermate control (Pml^+/+) and Pml^−/− mice were primed with LPS (0.5 μg/mL) (A), Pam3CSK4 (Pam3, 1 μg/mL) (B), or R837 (15 μg/mL) (C) for 4 hours, followed by incubation with ATP (4 mM) for 40 minutes (A-C), or MSU (150 μg/mL) for 4 hours (A). The secreted IL-1β in the supernatants was determined by ELISA. (D) Normal TNF-α secretion in PML-deficient macrophages. Bone marrow–derived macrophages from wild-type and Pml^−/− mice were stimulated with LPS or Pam3CSK4 for 6 hours, followed by ATP or nigericin (Nig, 10 μM), and secreted TNF-α was measured by ELISA. **P < .01; ***P < .001 for paired t-test. Data represent mean values and SD of a specific experiment with triplicate samples. All experiments were repeated 4 times with similar results.