Binding isotherms of the collagen-VWF interactions. Microtiter plates were coated with either type I (A) or type III (B) human collagen at a final concentration of 5 μg/mL and incubated with decreasing concentrations of wild-type or mutant recombinant VWF. As previously reported, H1786A shows no binding to either collagen type and only S1731T binds significantly to type 1 collagen.