Effects of reoxygenation on TREM-1 expression. Four-day H-mDCs were generated from different donors, CM was replaced with fresh medium supplemented with cytokines, and TREM-1 was analyzed after additional 24-hour culture under 20% O2 (Reox). (A) TREM-1 and CAXII mRNA expression was assessed by quantitative RT-PCR. Data from one representative experiment of 3 performed are expressed as in the legend to Figure 3A. (B) TREM-1 surface expression was evaluated by flow cytometry. Solid histograms represent the fluorescent profile of TREM-1-expressing cells; and open histograms, the fluorescent profile of cells stained with the isotype-matched control Ab. The percentage of TREM-1+ cells and the mean fluorescent intensity (in parentheses) are indicated. Results from one of 3 independently tested donors are shown. (C) sTREM-1 release was measured by ELISA. Data from one representative experiment of 3 performed are expressed as picograms/1 × 106 cells/mL.
