Complete green tea extract and its polyphenols EGC, ECG, and EC neutralize the cytotoxic effects of BZM. (A) Chemical structures of the green tea polyphenols EGC, ECG, and EC. (B) Percentage cell viability of LN229 cells treated for 48 hours with increasing concentrations of BZM in the presence of increasing concentrations of EGC, ECG, and EC was determined by MTT assay. Percentage cell viability of (C) LN229 and (D) U266 cells treated for 48 hours with increasing concentrations of BZM in the presence of increasing concentrations of complete green tea extract (GTE) was determined via MTT assay. In all cases, the average percentage of nontreated cell cultures was set to 100%; shown is mean plus or minus SE (n ≥ 4). (E) Typical result of MTT survival assay of RPMI/8226 cells treated for 48 hours with increasing concentrations of BZM in the presence of increasing concentrations of complete GTE. Shown are wells from a 96-well plate, and each condition is shown in quadruplicate. Deep purple color represents fully viable cell culture; pink(ish) color indicates reduced viability; yellow color indicates absence of viable cells. The concentration of EGCG noted in panels C through E was calculated based on the percentage of EGCG as listed on the label of the liquid GTE (“Materials”).