Evaluation of frequency of CFU-F from WT and Fancg^−/− MSPCs. (A) A representative photomicrograph of CFU-Fs from WT and Fancg^−/− BMMNCs, after staining with the HEMA-3 Quick Staining Kit. (B) Representative data of the quantitative frequency of CFU-Fs per tibia from WT and Fancg^−/− mice are shown (*P < .001 for comparison of WT and Fancg^−/− BMMNCs). (C) A representative photomicrograph of CFU-Fs from WT and Fancg^−/− BMMNCs cultured in hypoxic conditions. (D) Total CFU-Fs per tibia in hypoxia culture condition are shown. *P < .001 comparing Fancg^−/− with WT mice. Data are shown as mean ± SE of 5 independent experiments. The photomicrographs in panels A and C were taken (under 10× amplification) with a Nikon TE2000-S microscope (Nikon Instruments, Melville, NY), a QIMAGING camera, and QCapture Pro software (Fryer Company, Cincinnati, OH).