p120GFP overexpression increases VE-cad at the cell-cell junctions. HUVECs were infected with different doses of p120GFP (A,B), GFP (C), or VE-cadherin GFP (D). Hec-1–Alexa 568 Ab was used to detect VE-cad in each monolayer. The intensity of VE-cad at the junctions was quantified in live HUVECs by analyzing 20 cell-cell junctions including representative junctions of the heterogeneous population for each condition (in duplicate) for each experiment performed. (E) HUVECs were transduced with GFP or p120GFP (17.5 μL) surface biotinylated and lysed, or cultured for 2, 4, or 6 hours before being lysed and subjected to immunoprecipitation with Hec-1. A representative blot is shown. Values corresponding to the half-life were calculated according to the “exponential decay formula” and are the mean plus or minus SD of 3 different experiments.