DNA damage was assessed by γH2AX foci counting. (A) γH2AX foci are increased in 32D/ITD cells compared with 32D/WT and 32D cells. (B) γH2AX foci are increased in MOLM-14 and MV-4-11 cells (containing FLT3/ITD mutation) compared with REH (without FLT3/ITD mutation). Results of the foci counting are presented graphically showing the number of cells with fewer than 5 and more than 5 foci. (C) MV-4-11 cells were treated with 100 nM CEP-701 for 4 hours, followed by staining for γH2AX. Results are compared with those of untreated control. Left panel represents untreated and right panel represents CEP-701 treated cells. Graphic representation of foci showing a decrease in number of cells with more than 25 foci per cell in treated samples. The error bars represent plus or minus SEM.