Increased apoptosis and partial differentiation blockage of erythroblasts after palladin disruption. (A) Typical flow cytometric profiles of wt and Palld^−/− fetal liver single cells stained with CD71 and Ter119 are shown. Gates from R1 to R5 are set as indicated. (B) Comparison of wt and Palld^−/− fetal livers in the ratio of R1 to R5. (C) The ratio of annexin V–positive cells from R1 to R5 was compared in wt and Palld^−/− fetal livers. (D) Wt and Palld^−/− fetal liver single-cell cytospins were stained with Wright-Giemsa; arrows indicate the R3 population. Images were viewed with an Olympus BX51 microscope with an Olympus UplanFl 20×/0.50 objective, captured with a SPOT RTKE cooled color CCD camera (Diagnostic Instruments), and imported into SPOT software (Diagnostic Instruments). Note that Palld^−/− fetal liver has a decreased R3 population. Error bar represents plus and minus a SD; **P < .01; *P < .05. Original magnification, × 200.