Overexpression of MafB in BMMs attenuates expression of NFATc1 and OSCAR. BMMs were transduced with pMX-IRES-EGFP (control) or MafB retrovirus and cultured with M-CSF and RANKL for the indicated times. (A) Total RNA was collected from each time point. RT-PCR (top panel) and real-time PCR analysis (bottom panel) were performed to detect the indicated genes in control (▪) or MafB-overexpressed (□) samples (#P < .05 versus control vector; *P < .005 versus control vector). (B) Cells were harvested at each time point and lysates were analyzed by Western blot analysis using antibodies specific for MafB, NFATc1, OSCAR, and actin. Arrow indicates the band representing MafB.