Neutrophil recruitment in a model of LPS-induced lung inflammation and thioglycollate-induced peritonitis is partially dependent on Gα_i2 in neutrophils. (A) Neutrophil recruitment into the alveolar compartments of the lung with or without LPS inhalation (24 hours) was determined by flow cytometry. Gnai2^−/− mice, Gnai2^+/+ mice, and Gnai2^−/− → Gnai2^+/+ chimeric mice showed different recruitment patterns of neutrophils in the alveolar compartment (n = 4). Data are means plus or minus SEM. (B) Peritoneal neutrophil influx 4 hours after injection of 4% thioglycollate into Gnai2^−/− mice (4 mice), Gnai2^−/− mice (3 mice), Gnai2^−/− → Gnai2^+/+ chimeric mice (5 mice), and Gnai2^+/+ → Gnai2^+/+ mice (5 mice). Total number of neutrophils (× 10⁶) in the peritoneal cavity counted using Kimura-stained samples. Horizontal bars are means of 5 replicates. *P < .05 versus other groups; ^#P < .05.