Figure 1.
Phenotype of erythroleukemic cell lines and their sensitivity to TRAIL. (A) HeL, K562, and TF-1 cells were stained with anti-CD34, anti–glycophorin A, and with specific MoAbs to TRAIL-R1, TRAIL-R2, TRAIL-R3, and TRAIL-R4 as described in “Materials and methods.” Specific fluorescence histograms (gray) are superimposed to negative controls (empty histograms). (B) TRAIL-induced apoptosis of TF-1 cells treated for 48 hours with 50 ng/mL TRAIL. Apoptosis was detected by staining cells with annexin V–FITC and PI.