Figure 2.
![Figure 2. hMSCs inhibit B-cell proliferation through the release of soluble factors. hMSCs and B cells at a 1: 1 ratio were seeded in 24-well plates on the opposite sides of a 5-μm pore-size polycarbonate membrane (transwell) and cultured for 3 days. Aliquots of the same hMSC and B-cell suspensions were cocultured at the same ratio for the same time. Control B cells were cultured alone. Stimuli were CpG 2006, rCD40L, anti-immunoglobulin, IL-2, and IL-4. B-cell proliferation was assessed by [3H]-thymidine incorporation. Results are expressed as median counts per minute, maximum and minimum values from 5 different experiments. **P = .009.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/1/10.1182_blood-2005-07-2657/4/zh80010688560002.jpeg?Expires=1754652994&Signature=4Wn-riVsnrDrNF2kq8yzN7bmiUt07uW-B92rIXxnob4PyuZbVzECBT1MfJvJim8U22Bt0-gAcg4-u7B6g5f52fXMbQjtNNJeOkgRo8rvl~pdFUtTmwqA6nI4lzi2RqA4YGtjQ4~-NkWGTxEm35J9tbpdC5nwmyuKfvcHwZdcqqK37ajtXFrgFI8FlKc3~ci1x2B-eZN6LbsKcC7jj27pRJyerTuuxpF13CPKe3EdpfucAvo1BUZ7jaUyw3trXOv3y9EEhDoBBD2Shvlo~Q6LvuUMZEOIFT9XoX3Hf~DGMOAw8eaH7WyYzIUm0yunzAUN0rmxvFBC0b500GjUDbTX8g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
hMSCs inhibit B-cell proliferation through the release of soluble factors. hMSCs and B cells at a 1: 1 ratio were seeded in 24-well plates on the opposite sides of a 5-μm pore-size polycarbonate membrane (transwell) and cultured for 3 days. Aliquots of the same hMSC and B-cell suspensions were cocultured at the same ratio for the same time. Control B cells were cultured alone. Stimuli were CpG 2006, rCD40L, anti-immunoglobulin, IL-2, and IL-4. B-cell proliferation was assessed by [3H]-thymidine incorporation. Results are expressed as median counts per minute, maximum and minimum values from 5 different experiments. **P = .009.
