MV-Edm targeted to the CD38 receptor via αCD38.

(A) Schematic representation of the full-length infectious clone of recombinant MV-Edm targeted to the CD38 receptor via a single-chain antibody (αCD38). The single-chain antibody is displayed as a C-terminal extension on the H glycoprotein. * indicates stop codons. (B) Immunoblot of purified MV-αCD38 and MV-Edm virions. The recombinant H glycoprotein of MV-αCD38 (lane 1) has a higher molecular weight compared with that of MV-Edm (lane 3). After treatment with Factor Xa protease (20 μg/mL), the single-chain antibody was cleaved off, leaving a H glycoprotein (lane 2) that has the same molecular size as MV-Edm (lane 4).