Fig. 5.
Fig. 5. Effects of the membrane form of SCF on the mast cell expression of Gi3α protein. / SDS-PAGE immunoblot (A) and confocal microscopy images of Gi3α protein under orange fluorescence (FITC) were performed with CBMCs maintained in the presence of soluble SCF (100 ng/mL) and cocultured for 4 days with 3T3 fibroblasts previously treated overnight with 0.1 pM SCF sense (B) or SCF antisense (C). The displayed figures are from a representative single experiment of 3. Original magnification B-C, × 63.

Effects of the membrane form of SCF on the mast cell expression of Gi3α protein.

SDS-PAGE immunoblot (A) and confocal microscopy images of Gi3α protein under orange fluorescence (FITC) were performed with CBMCs maintained in the presence of soluble SCF (100 ng/mL) and cocultured for 4 days with 3T3 fibroblasts previously treated overnight with 0.1 pM SCF sense (B) or SCF antisense (C). The displayed figures are from a representative single experiment of 3. Original magnification B-C, × 63.

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