Fig. 1.
Fig. 1. Loss of naive phenotype after transduction of T cells with HIV-1–derived vectors displaying a CD3/TCR-stimulating ligand. / (A) Freshly isolated total human peripheral blood lymphocytes (PBLs) or (B) highly purified naive adult CD4+ T cells (> 98% CD45RA+) were transduced with anti-CD3 scFv-displaying lentiviral vectors pseudotyped with VSV-G (G/OKT3SU) in the absence of other exogenous factors using MOIs of 10 to 15. Control PBLs and naive T cells were transduced with VSV-G–pseudotyped lentiviral vectors (G) in the presence of soluble anti-CD3 and anti-CD28 monoclonal antibodies (1 μg/mL). The percentage of EGFP+ and naive T cells (CD45RA+) was determined 6 days after transduction by FACS analysis. The results are representative of 5 independent experiments.

Loss of naive phenotype after transduction of T cells with HIV-1–derived vectors displaying a CD3/TCR-stimulating ligand.

(A) Freshly isolated total human peripheral blood lymphocytes (PBLs) or (B) highly purified naive adult CD4+ T cells (> 98% CD45RA+) were transduced with anti-CD3 scFv-displaying lentiviral vectors pseudotyped with VSV-G (G/OKT3SU) in the absence of other exogenous factors using MOIs of 10 to 15. Control PBLs and naive T cells were transduced with VSV-G–pseudotyped lentiviral vectors (G) in the presence of soluble anti-CD3 and anti-CD28 monoclonal antibodies (1 μg/mL). The percentage of EGFP+ and naive T cells (CD45RA+) was determined 6 days after transduction by FACS analysis. The results are representative of 5 independent experiments.

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