Expression of ras, large T, or hTERT in the transduced human stromal cell lines.

Expression of the transgene was analyzed by immunoblot analysis (panel A), by RT-PCR (panel B), or by the level of telomerase activity (panel C). (A) Lane 1, primary stromal cells; lane 2, large T–stromal cells; lane 3, ras-stromal cells; lane 4, hTERT-stromal cells. Anti–large T indicates primary antibody for SV40 large T protein; anti-Ras, antibody for H-ras protein; anti-hTERT, antibody for hTERT protein; anti–β-actin, antibody specific for β-actin protein, as an internal standard. (B) Lane 1, primary stromal cells; lane 2, large T–stromal cells; lane 3, ras-stromal cells; lane 4, hTERT-stromal cells. RT-PCR was performed for the integrated hTERT provirus sequence and the β-actin sequence as an internal standard. (C) Lane 1, human vascular endothelial cells (HUVECs); lane 2, Hela cells; lane 3, primary stromal cells; lane 4, large T–stromal cells; lane 5, ras-stromal cells; lane 6, hTERT-stromal cells.