Effects of AG490, PD98059, SB203580, and wortmannin on expression of cIAP1 and cIAP2 mRNA in neutrophils stimulated by G-CSF.

Neutrophils were preincubated with AG490 (50 μM), PD98059 (50 μM), SB203580 (10 μM), or wortmannin (100 nM) for 20 minutes, and thereafter cultivated in the presence or absence of G-CSF (50 ng/mL) for 1 hour. The expression of cIAP1 and cIAP2 mRNA was analyzed by real-time PCR. As an internal control, 18S rRNA was used. The ratios of cIAP1 to 18S rRNA and cIAP2 to 18S rRNA were calculated, and the ratio at hour 0 is expressed as 1. The data are expressed as means ± standard errors of the mean of 3 to 5 experiments. *Significantly inhibited by AG490 (P < .05).