Fig. 2.
Fig. 2. Activation of various Val814 mutants with substitutions of Tyr→Phe and deletion of C-terminus (Del). / Various Val814 mutants were immunoprecipitated with an ACK2 mAb from the lysates of transfected 293T cells and were subjected to the immune complex kinase assay (upper lanes) and immunoblotting with a rabbit polyclonal Ab against the whole murine KIT (lower lanes). Not all samples were stimulated with rmSCF. Similar results were obtained from 3 independent experiments. Y represents tyrosine (Tyr); F, phenylalanine (Phe).

Activation of various Val814 mutants with substitutions of Tyr→Phe and deletion of C-terminus (Del).

Various Val814 mutants were immunoprecipitated with an ACK2 mAb from the lysates of transfected 293T cells and were subjected to the immune complex kinase assay (upper lanes) and immunoblotting with a rabbit polyclonal Ab against the whole murine KIT (lower lanes). Not all samples were stimulated with rmSCF. Similar results were obtained from 3 independent experiments. Y represents tyrosine (Tyr); F, phenylalanine (Phe).

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