Results of Q-PCR in PBMCs and plasma.
Detection of HHV-6 DNA by quantitative real-time PCR in samples from PBMCs (A) and plasma (B) after stem cell transplantation. The viral load value was classified according to the days after transplantation as day 0 to 6, 7 to 13, and so forth. The solid and broken lines represent the change in median viral load in CBSCT and BMT/PBSCT procedures, respectively, at each period. The maximum and minimum values are shown by the upper and lower bars. Each closed circle (●) or open box (■) below the dotted line represents a sample from CBSCT or BMT/PBSCT recipients that was under the detectable limit (< 80 copies/105 PBMCs and < 20 copies/25 μL plasma), respectively. The number of positive and total samples that were subjected to Q-PCR assay is shown, with the positive rates observed at each period.
