Generation and characterization of Mushi Tg mouse lines. (A) Schematic representation of the Tg construct. The Tg consists of the mouse CD11c promoter, the intron and the polyA addition signal from the rabbit β1 globin gene, the gene coding for SV40 T oncogenes, and a second cistron for eGFP. FRT and loxP sites were added to allow generation of single-copy Tg mice and to remove the expression cassette, respectively. Primers (arrows) flanking the introns of rabbit β1 globin and SV40 T oncogenes were used for screening and to monitor splicing of Tg transcripts. (B) Detection of Tg spliced transcripts coding for SV40 large T and small T antigens by RT-PCR (as described in A) on spleen of the independent Tg lines Mushi1 and Mushi2, littermates (LMs), control genomic Tg DNA (DNA TG), and negative control (TE). (C) FACS analysis of Tg expression. CD11c and GFP levels in spleen Tg Mushi1 and Mushi2 and non-Tg littermate (LM) cells.