MLR T cell populations are sorted by FACS (A). Clonotypic compositions of rapid responder (RR), slow responder (SR), and non-responder (NR) cells are determined (B). Candidate AR/GVL clonotypes are validated bioinformatically (C). Three representative patient courses demonstrate MRD quantification (red) and presence of AR/GVL clonotypes (black) in a patient with late relapse, absence in a patient with early relapse, and clonotypic transition in a patient with post-DLI GVHD (D).