(A) Cytostatic activity of SK053 in established human AML cell lines evaluated with trypan blue staining, the graph presents mean cell count ± SD, n=6 (B) Differentiation of AML primary blast after treatment with SK053. Cells pre-incubated for 3 days with SK053 were incubated with fluorochrome-conjugated anti-CD11b mAb for 30 min. at RT in the dark. Subsequently dead cells were stained with 7AAD. Graphs represent MFI only from 7AAD^-CD33⁺population, n=3, *P<0.05 vs controls, one-way ANOVA with Dunett’s post test (C) Knock-down of PDI, TRX 1 and PRDX 1 in HL60 cells do not result in granulocytic differentiation. 10 days post transduction with lentiviral shRNA systems HL60 cells were stained for CD11b marker as described above. Graphs represent MFI only from 7AAD^- cells.