Figure 3.
Yolk Sac Myeloid Progenitors from PTPN11D61Y/+; VavCre+ embryos demonstrate Ras-Erk pathway hypersensitivity at baseline and following GM-CSF stimulation. Cultured yolk sac progenitors were stimulated for 30min with 5ng/ml of GM-CSF, processed for intracellular flow cytometry, and stained with the indicated fluorescent antibody. Histograms display representative median fluorescence intensity among CD45+ cells in each sample (n=3). * p<0.05 by two-tailed Student’s t-test. ADDIN EN.REFLIST
