Generation and Initial Characterization of Cbl^Y371H Transgenic Mice A.Flow cytometry of splenocytes from the various transgenic lines. Panel A demonstrating tdTomato fluorescence from each of the transgenic lines generated. Note that there are expression differences among the 6 lines with line L5 expressing the highest amount of fluorescence and line L6 with no fluorescence. B.Western Blot of spleen extracts from transgenic mice Panel B confirms human Cbl protein expression from transgenic mice. L5 and L6 lines express the most protein followed by K5 and D7 as predicted based on Tdtomato expression in panel A except L6 C.Genotyping of mice for cbl ko and cbl transgene As seen in the figure, Cbl PCR and Cbl KO PCR is done separately with #130 showing that it carries the transgene for Cbl as well as being homozygous for Cbl KO allele.