HSCs are exclusively driving multi-lineage engraftment and allow prediction of neutrophil and platelet engraftment in the NHP transplant model.

(A) Hierarchical organization of HSPCs in the NHP. HSPC of group I, II, and III were sort-purified and transduced with LV vectors expressing either green fluorescent protein (GFP; population I), mCherry (population II) or mCerulean (population III), respectively. (B) Long-term follow-up of gene modified white blood cell (WBC) levels in vivo after myeloablative transplantation of these cell populations. (C and D) Correlation of transplanted HSCs/kg body weight with (C) neutrophil and (D) platelet recovery. Animals demonstrating engraftment failure (red squares) were excluded from this correlation.