Mutational analysis. Graphic representation of number of samples analyzed for each patient (N = 112) and mutations observed in the first sample analyzed. Green box, sample on whom TED profile was obtained; red box, the sample was adequate but no cells were found undergoing TED. Each column represents 1 unique patient. Although for some patients, every sample analyzed at different time points showed TED, there were others who failed to show cells in TED at every point analyzed; there were some samples that failed to show cells in TED at 1 or more point. Colored boxes colored according to their functional group in mutation panels show presence of mutation in that gene and patient. Splicing factor mutations were more common followed by DNA methylation. Bottom, color codes for the IPSSR and WHO categories.