Figure 1.
Figure 1. Molecular studies of the GATA1 gene. The GATA1 gene was amplified using gDNA and cDNA genomic DNA and cDNA. (A) PCR amplified products of GATA1 gene using 8 overlapping primer sets. (B) RT-PCR amplified products of exon 2 of GATA1, GATA1 mRNA (400 bp), and GATA1s mRNA (172bp) are shown. (C) Reverse partial chromatogram of GATA1 gene in exon 2. Direct sequencing data showed GATA1 mutations from 109–110 del AG in exon 2 in patient 1 with TAM and patient 6 with AMKL, 1–239del239bp in patient 2 with TAM, 20A>G in patient 3 with TAM, and 193ins19bp in patient 5 with AMKL.

Molecular studies of the GATA1 gene. The GATA1 gene was amplified using gDNA and cDNA genomic DNA and cDNA. (A) PCR amplified products of GATA1 gene using 8 overlapping primer sets. (B) RT-PCR amplified products of exon 2 of GATA1, GATA1 mRNA (400 bp), and GATA1s mRNA (172bp) are shown. (C) Reverse partial chromatogram of GATA1 gene in exon 2. Direct sequencing data showed GATA1 mutations from 109–110 del AG in exon 2 in patient 1 with TAM and patient 6 with AMKL, 1–239del239bp in patient 2 with TAM, 20A>G in patient 3 with TAM, and 193ins19bp in patient 5 with AMKL.

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