Figure 1 Foxp3 expression in CD4 CD25 −... | American Society of Hematology

Figure 1

$Figure 1. Foxp3 expression in CD4+CD25− T cells in biopsy specimens of B-cell NHL. (A) Representative dot plots showing Foxp3 expression in CD4+CD25+ and CD4+CD25− T cells in biopsy specimens of patients with NHL. Intracellular Foxp3 expression was determined by using fluorochrome-conjugated Foxp3 antibody. (B) Percentage of Foxp3 expression in different subsets of cells in NHL specimens (n = 12). The horizontal bars indicate median expression levels. (C) Representative dot plots showing Foxp3 expression in positive (CD25+) and negative (CD25−) fractions of CD25-depleted sample. (D) Representative immunofluorescence images showing Foxp3 expression in CD25+ and CD25− cells in biopsy specimens of patients with NHL. Tissues were stained with antibodies to human CD25 and Foxp3. Left panels shows isotype control for each antibody; right, double immunofluorescence staining of CD25 (red; surface) and Foxp3 (green; intracellular). Original magnification, × 200. See “Immunofluorescence assay” for image acquisition details. (E) Representative dot plots showing Foxp3 expression in CD4+CD25− T cells (CD25-depleted) from tumor tissue (top panel) and peripheral blood mononuclear cells (bottom panel) from patients with NHL. Percentages in panels A,C, and E represent number of cells in quadrant as a percentage of all cells.$

Foxp3 expression in CD4⁺CD25⁻ T cells in biopsy specimens of B-cell NHL. (A) Representative dot plots showing Foxp3 expression in CD4⁺CD25⁺ and CD4⁺CD25⁻ T cells in biopsy specimens of patients with NHL. Intracellular Foxp3 expression was determined by using fluorochrome-conjugated Foxp3 antibody. (B) Percentage of Foxp3 expression in different subsets of cells in NHL specimens (n = 12). The horizontal bars indicate median expression levels. (C) Representative dot plots showing Foxp3 expression in positive (CD25⁺) and negative (CD25⁻) fractions of CD25-depleted sample. (D) Representative immunofluorescence images showing Foxp3 expression in CD25⁺ and CD25⁻ cells in biopsy specimens of patients with NHL. Tissues were stained with antibodies to human CD25 and Foxp3. Left panels shows isotype control for each antibody; right, double immunofluorescence staining of CD25 (red; surface) and Foxp3 (green; intracellular). Original magnification, × 200. See “Immunofluorescence assay” for image acquisition details. (E) Representative dot plots showing Foxp3 expression in CD4⁺CD25⁻ T cells (CD25-depleted) from tumor tissue (top panel) and peripheral blood mononuclear cells (bottom panel) from patients with NHL. Percentages in panels A,C, and E represent number of cells in quadrant as a percentage of all cells.

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