Figure 6 Dok-3 deficiency leads to less... | American Society of Hematology

Figure 6

$Figure 6. Dok-3 deficiency leads to less sustained SHIP-1 activation after BCR stimulation. (A) Western blot analysis of whole-cell lysates obtained from purified BCR-stimulated wild-type and Dok-3−/− B cells using anti-SHIP-1 and antiphospho-SHIP-1 antibodies. Data shown are representative of 5 independent experiments. (B) Analyses of SHIP-1 recruitment to plasma membrane after BCR stimulation in Dok-3−/− B cells. Plasma membrane fractions were obtained from wild-type and mutant B cells at various time points after BCR engagement and probed for the presence of phospho-SHIP-1 and SHIP-1 proteins. The anti-Lyn blot was included as control for equal loading of plasma membrane fractions. Data shown are representative of 3 independent experiments.$

Dok-3 deficiency leads to less sustained SHIP-1 activation after BCR stimulation. (A) Western blot analysis of whole-cell lysates obtained from purified BCR-stimulated wild-type and Dok-3^−/− B cells using anti-SHIP-1 and antiphospho-SHIP-1 antibodies. Data shown are representative of 5 independent experiments. (B) Analyses of SHIP-1 recruitment to plasma membrane after BCR stimulation in Dok-3^−/− B cells. Plasma membrane fractions were obtained from wild-type and mutant B cells at various time points after BCR engagement and probed for the presence of phospho-SHIP-1 and SHIP-1 proteins. The anti-Lyn blot was included as control for equal loading of plasma membrane fractions. Data shown are representative of 3 independent experiments.

This Feature Is Available To Subscribers Only