Enhanced NFκB, JNK and p38 MAPK activation in BCR-activated Dok-3^−/− B cells. (A) Enhanced NFκB activation in BCR-stimulated Dok-3^−/− B cells. Purified splenic B cells were stimulated via their BCR and the activation of NFκB was assessed via degradation of IκBα in Western blot analysis (upper panel) and induction of DNA-binding activity in gel-shift assay (lower panel). The anti-ERK2 blot was included as control for equal loading of whole cell lysates in the Western blot analysis. Equal amount of nuclear extracts as determined by BCA assay was used in the EMSA (electrophoretic mobility shift assay). Data shown is representative of 3 experiments. (B) Enhanced activation of JNK and p38 MAPK signaling in BCR-stimulated Dok-3^−/− B cells. Purified splenic B cells were stimulated via their BCR and examined for various MAPK activation using specific antibodies that recognized the phosphorylated forms of the various MAPKs. Anti-ERK2, anti-JNK1, and anti-p38 blots were included as controls for equal loading of cell lysates. Figure shown is representative of at least 3 independent experiments.