Dok-3−/− B cells are hyperresponsive to BCR stimulation in vitro. (A) Purified wild-type (white column) and Dok-3−/− (black column) splenic B cells were not treated or treated with anti-IgM F(ab′)2 alone or with anti-IgM F(ab′)2 and CD40 ligand and the proliferation of cells was measured via incorporation of thymidine. Statistical significance was determined by paired 2-tailed Student t test. Figure shown is representative of more than 3 independent experiments. (B) Enhanced calcium signaling in Dok-3−/− B cells. Purified wild-type (gray line) and Dok-3−/− (black line) B cells were stimulated with either 2 or 20 μg/mL of anti-IgM or anti-IgG antibodies and the elicited intracellular calcium flux was monitored in real time via flow cytometry. Figure shown is representative of 2 independent experiments.
