Figure 4
CD34 expression on UCB-derived CD34+ progenitors cultured in the presence of SCF, FLT3L, TPO in 2% to 3% versus 20% O2. FACS staining for surface CD34 was performed on cells cultured for 48 hours in 2% to 3% versus 20% O2 on PLL- versus FN-coated plates. (A) Mean percentage of CD34+ cells ± SEM after culture at 20% on FN (white bar), 2% to 3% on PLL (gray bar), and 2% to 3% on FN (black bar) from 3 independent experiments based on ungated (left) versus gated on live cells (right). (B) We show a representative FACS analysis; analysis was gated on live cells only.
