Effect of GILZ in GC-treated patients. Patients were tested before and after 48 hours of treatment with GCs. Their APCs were transfected with control (siC; □) or GILZ (siGILZ; ▪) siRNA, loaded with PPD, and cocultured with autologous CD4⁺ T lymphocytes. Proliferation (A,C) and IFNγ production (B) were determined on day 7 of culture. (A-B) Results are expressed as means ± SEM for 4 patients; (C) a typical experiment.