Figure 4.
Figure 4. GILZ effect on DC maturation. (A-B) MoDCs were nucleofected with either pcDNA3 or pGILZ and stimulated with CD40L or left unstimulated, creating mature DCs (mDCs) and immature DCs (iDCs), respectively. (A) CD80 and CD83 expression was analyzed in DCs transfected with pcDNA3 (bold lines) or pGILZ (gray histograms). (B) phagocytosis of FITC-Dx was analyzed at 37°C (bold lines) or at 4°C (gray histograms). (C-D) CD34-DCs were infected with LvGILZ or LvTK on day 8 of culture. CD40L was added or not on day 10. Results shown are gated on CD90+ (transduced) DCs. (C) DC phenotype was determined on day 12. Bold lines and gray histograms correspond to LvTK- and LvGILZ-infected DCs, respectively. (D) Phagocytosis of FITC-Dx was determined at 37°C (bold lines) or 4°C (gray histograms). Results are from 1 representative experiment of 3.

GILZ effect on DC maturation. (A-B) MoDCs were nucleofected with either pcDNA3 or pGILZ and stimulated with CD40L or left unstimulated, creating mature DCs (mDCs) and immature DCs (iDCs), respectively. (A) CD80 and CD83 expression was analyzed in DCs transfected with pcDNA3 (bold lines) or pGILZ (gray histograms). (B) phagocytosis of FITC-Dx was analyzed at 37°C (bold lines) or at 4°C (gray histograms). (C-D) CD34-DCs were infected with LvGILZ or LvTK on day 8 of culture. CD40L was added or not on day 10. Results shown are gated on CD90+ (transduced) DCs. (C) DC phenotype was determined on day 12. Bold lines and gray histograms correspond to LvTK- and LvGILZ-infected DCs, respectively. (D) Phagocytosis of FITC-Dx was determined at 37°C (bold lines) or 4°C (gray histograms). Results are from 1 representative experiment of 3.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal