Comparison of APCE with rFAP for molecular size and gelatinase activity. A protein load of 1.4 μg was applied to each electrophoretic lane. Each was stained with Coomassie brilliant blue G-250. Clear zones are due to gelatinase activity. (A) Electrophoresis in the presence of SDS on polyacrylamide gel containing copolymerized gelatin. (B) Electrophoresis in the absence of SDS, but on the identical type of gel. Comparable gelatinolysis is observed for the 2 enzymes; however, the mobilities of the single broad zones of lysis differ slightly and cannot be related to molecular weight standards because electrophoresis was done without SDS. (C) Analytical size exclusion chromatography of APCE (blue) and rFAP (red). Standard (black): a, thyroglobulin (670 kDa); b, γ-globulin (158 kDa); c, ovalbumin (44 kDa); and d, myoglobin (17 kDa). The inset shows a standard plot of the logarithmic molecular weight against the elution volume (Ve)/void volume (Vo).