Treg are effectively depleted ex vivo with CD25 microbeads and the Clinimacs device. A FACS plots showing frequency of CD4 ⁺CD25 ⁺ Tregs before (left) and after (right) ex vivo Treg depletion procedure (arm 3). B Mean CD4+CD25+ Treg frequencies before and after ex vivo Treg depletion (*p= 0.05).