Figure 5.
ACL activity determines the states of giant cells. (A) Acetyl-CoA analysis in parental and giant cells treated with or without ACLi (BMS303141) at 10 μM for 48 hours. ∗P < .05. (B) OCR in Mino-VEN-R giant cells treated with or without ACLi (10 μM for 16 hours). (C) Representative transmission electron microscopy imaging of giant cells treated with or without ACLi (10 μM for 48 hours) and acetate supplement (20 mM for 48 hours). Lipid droplet (red arrow) and mitochondria (yellow arrow). Scale bar, 200 nm. (D) Representative imaging showing giant cells reproliferated after drug removal treated with ACLi (10 μM; 9 days); acetate supplement (20 mM) prevented giant cells from reproliferating. Scale bar, 100 μm. (E) GSEA of pathways in P, G1, R1, and G1 treated with ACLi, and G1 treated with ACLi (10 μM) with acetate supplement (20 mM for 48 hours). (F) Western blots showing globally increased acetylation of nonhistone proteins in giant cells. Ponceau S staining shows total protein levels. NES, normalized enrichment score; Pval, P value.

ACL activity determines the states of giant cells. (A) Acetyl-CoA analysis in parental and giant cells treated with or without ACLi (BMS303141) at 10 μM for 48 hours. ∗P < .05. (B) OCR in Mino-VEN-R giant cells treated with or without ACLi (10 μM for 16 hours). (C) Representative transmission electron microscopy imaging of giant cells treated with or without ACLi (10 μM for 48 hours) and acetate supplement (20 mM for 48 hours). Lipid droplet (red arrow) and mitochondria (yellow arrow). Scale bar, 200 nm. (D) Representative imaging showing giant cells reproliferated after drug removal treated with ACLi (10 μM; 9 days); acetate supplement (20 mM) prevented giant cells from reproliferating. Scale bar, 100 μm. (E) GSEA of pathways in P, G1, R1, and G1 treated with ACLi, and G1 treated with ACLi (10 μM) with acetate supplement (20 mM for 48 hours). (F) Western blots showing globally increased acetylation of nonhistone proteins in giant cells. Ponceau S staining shows total protein levels. NES, normalized enrichment score; Pval, P value.

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