Neutrophils associated with FLs acquire a protumor phenotype. (A) RNA velocity of the reanalyzed neutrophil data set excluding macrophages. UMAP embeddings from 84 616 cells. Color convention as per Figure 1A. The visual representation of neutrophil maturation from HBM (left), NDMM (middle), and RRMM (right). Scaled bar graphs below HBM and patient-specific UMAP represent scaled contribution of immature (gray) and mature (blue) neutrophils. (B) UMAP representation of neutrophils observed. The contribution of BM cells (n = 49 016 cells) is shown in blue. The contribution of FL cells (n = 35 600 cells) is shown in red. (C) Clusters scored against mature neutrophil signature (see “Materials and methods” for details). High scores represent transcripts within clusters most like mature neutrophils. (D) Highly variable features associated with neutrophil maturity in single cell data. Data are organized by early to late neutrophil development. Blue indicates BM cells, and red indicates FL cells. (E) Differentially expressed transcripts in neutrophils between BM (left, blue) and FLs (right, red). Features were selected to emphasize proinflammatory or immunosuppressive phenotypes. (F) Paired gene ontogeny analysis of mature neutrophils from FLs vs neutrophils from HBM.