Neutrophils from patients with AML display maturation impairment and express CD14. (A) Bidimensional UMAP analysis of 68 214 CD45⁺ cells from healthy and AML-diagnosed fresh patient blood samples (n = 17). (B) Expression of CD15⁺ CD66B⁺ neutrophils (blue, healthy; red, AML). (C) Five hundred neutrophils from each human sample were projected onto a new bidimensional UMAP. (D) Surface expression of CD14 on neutrophils (blue, healthy; red, AML). (E) Surface expression of CD101 on neutrophils (blue, healthy; red, AML). (F) Surface expression of CD10 on neutrophils (blue, healthy; red, AML). (G,J) Representative histogram and quantification of expression of CD14 in healthy and patient neutrophils (n > 10). (H,K) Representative histogram and quantification of expression of CD101 in healthy and patient neutrophils (n > 10). (I,L) Representative histogram and quantification of CD10 expression in healthy and patient neutrophils (n > 10). Significance is denoted as ∗∗∗∗P ≤ .0001; ∗∗P ≤ .01.