NF-κB–driven genes are upregulated in AML-exposed neutrophils. (A) Bidimensional t-SNE analysis of 30 215 neutrophils from ex vivo cultures separated into the 3 conditions: G-CSF, GM-CSF, and GMD. (B) Venn diagram representing DEGs in the GMD vs GM-CSF conditions. (C) Volcano plot of DEGs upregulated in GMD conditions (441 genes; blue) or GM-CSF conditions (540 genes; red). (D) Gene ontology analysis of DEGs in panel C. (E) Score of NF-κB–dependent genes in healthy (blue) and AML-exposed neutrophils (red). (F) Inflammation score of healthy neutrophils, AML-exposed neutrophils, and neutrophils from ex vivo cultures. (G) Degranulation score of healthy neutrophils, AML-exposed neutrophils, and neutrophils from ex vivo cultures. (H) Monocyte gene score of healthy neutrophils, AML-exposed neutrophils, and neutrophils from ex vivo cultures. (I) Venn diagram representing DEGs in AML-exposed neutrophils and GM-CSF conditions. (J) Venn diagram representing DEGs in AML-exposed neutrophils and G-CSF conditions. All DEGs have fold change >1.5; P ≤ .05. Significance is denoted as ∗∗∗∗P ≤ .0001 (1-way ANOVA analysis in panels E-H). Cntrl, control.