C1, C2, and C3 neutrophil clusters in AML are more immature than their healthy counterparts and negatively corelate with disease progression. (A) Heat map displaying normalized mean fluorescence intensity (MFI) of various neutrophil markers Ly6G, CD101, CXCR2, CD62L, and SSC in all 3 neutrophil clusters in the BM of AML and healthy mice (n > 9). (B) Ly6G and SSC MFI quantified in BM neutrophils (n > 9). (C) Representative fluorescence-activated cell sorter plot displaying BM neutrophils quantified by SSC, Ly6G, CXCR2, and CD101. (D) Heat map displaying normalized MFI of various neutrophil markers Ly6G, CD101, CXCR2, CD62L, and SSC in neutrophil cluster C3 in the blood of AML and healthy mice (n = 8). (E) CD101 and SSC MFI quantified in blood neutrophils (n = 8). (F) Simple linear regression displaying negative correlation between WBC count and frequency of CD101+ neutrophils in the blood in AML (n > 10). (G) Simple linear regression displaying negative correlation between WBC count and frequency of CD62L+ neutrophils in the blood in AML (n > 10). Data are shown as mean ± standard deviation. Significance is denoted as ∗∗∗∗P ≤ .0001, not significant (ns) P > .05, calculated using 1-way ANOVA (B) and unpaired 2-tailed t test (E); and P ≤ .05 for simple linear regression test (panels F-G).
Figure 2.

C1, C2, and C3 neutrophil clusters in AML are more immature than their healthy counterparts and negatively corelate with disease progression. (A) Heat map displaying normalized mean fluorescence intensity (MFI) of various neutrophil markers Ly6G, CD101, CXCR2, CD62L, and SSC in all 3 neutrophil clusters in the BM of AML and healthy mice (n > 9). (B) Ly6G and SSC MFI quantified in BM neutrophils (n > 9). (C) Representative fluorescence-activated cell sorter plot displaying BM neutrophils quantified by SSC, Ly6G, CXCR2, and CD101. (D) Heat map displaying normalized MFI of various neutrophil markers Ly6G, CD101, CXCR2, CD62L, and SSC in neutrophil cluster C3 in the blood of AML and healthy mice (n = 8). (E) CD101 and SSC MFI quantified in blood neutrophils (n = 8). (F) Simple linear regression displaying negative correlation between WBC count and frequency of CD101+ neutrophils in the blood in AML (n > 10). (G) Simple linear regression displaying negative correlation between WBC count and frequency of CD62L+ neutrophils in the blood in AML (n > 10). Data are shown as mean ± standard deviation. Significance is denoted as ∗∗∗∗P ≤ .0001, not significant (ns) P > .05, calculated using 1-way ANOVA (B) and unpaired 2-tailed t test (E); and P ≤ .05 for simple linear regression test (panels F-G).

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