AML-exposed neutrophils undergo altered maturation. (A) Experimental design of the Meis1-HoxA9 AML mouse model. (B) Representative confocal imaging of femur cross-sections in control and AML mice displaying laminin and neutrophils (CatchUp). (C-D) Representative gating strategy for C1, C2, and C3 neutrophils in BM (C) and blood (D) in AML. (E) Quantification of neutrophil frequency as a percentage of total CD45 cells in the BM (n > 10). (F) Simple linear regression displaying negative correlation (R2 = –0.52; P ≤ .05) between WBC count and frequency of neutrophils as a percentage of total CD45 cells in the blood in our AML mouse model (n > 10). (G) Quantification of C1, C2, and C3 neutrophil subpopulations in healthy and AML mice in the BM and blood (n > 10). Data are shown as mean ± standard deviation. Significance is denoted as ∗∗∗∗P ≤ .0001, calculated using unpaired 2-tailed t test (E), simple linear regression test (F), and 2-way analysis of variance (ANOVA) (G). TX, transplant.
