Inhibition of G9a or DNMTs promotes upregulation of TSGs in MM cells. (A) Average DNA methylation levels of TSGs from AZA-treated INA-6 MM cells (n = 3). Statistical analysis was performed with multiple t tests, corrected for multiple comparisons with the Holm-Šídák method. (B) RNA-seq data of TSGs gaining expression in AZA-treated INA-6 MM cells (n = 3). Statistical analysis was performed with multiple t tests. Values presented with SEM. (C) Upregulation of the TSGs MME, APAF1, and TTN in response to G9ai in MM cells (n = 2-3). Statistical analysis was performed with multiple t tests. Values presented with SEM. (D-H) Normalized expression of G9a/DNMT-associated TSGs in patients with MM compared to tPCs from the BPC data set (n_MM = 3; n_tPC = 3). Statistical analysis was performed with Student t test. Values are presented with SEM. (I-M) Normalized (MAS5) TTN, MME, APAF1, BLM, and LIMA1 expression-based survival data from BM-collected CD138⁺ from patients with MM (GSE9782). The analysis was performed on GenomicScape using log-rank test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. DMSO, dimethyl sulfoxide.