GPCR signaling is a common enriched pathway in liver of hemolytic and SCD mice. (A) Venn diagram plot showing the altered pathways determined from GSEA; altered pathway between PHZ-treated vs PBS-treated mice is in brown; and the altered pathway between HbSS vs HbAA mice is in green. The common altered pathways appear in yellow. (B-C) Bubble plot showing the absolute NES of the common altered pathways, highlighting the GPCR signature in PHZ- vs PBS-treated mice (B) and HbSS vs HbAA mice (C). The pathways enriched in PHZ and HbSS are shown in red, and the pathways enriched in their control counterpart are shown in blue. (D-E) Gene set enrichment score of the GPCR ligand binding pathway in PHZ vs PBS mice (D) and HbSS vs HbAA mice (E). FDR qval, false discovery rate q-value; NES, normalized enrichment score.