sgRNA CRISPR screen highlights key regulators of SM response, including SP1 and acetylation-modulating enzymes. (A) Schematic representation of experimental setup. (B) Volcano plot displaying significantly enriched or depleted sgRNAs (cutoffs: |robust rank aggregation [score]| > 0.495; FDR < 0.05; left). sgRNA rank plot depicting enriched (red) or dropout (blue) sgRNAs for the genes shown with the volcano plot plus intergenic controls (right). Each line represents 1 sgRNA; total sgRNAs per gene are 12 (4 sgRNAs from the Brunello library, 3 replicates). (C) Transcription frequency in TPM of sgRNAs for genes of interest. RIPK1 is included as a positive control. One dot represents 1 replicate. TPM, transcript per million.