MIRCA device, assay schematic, and readouts. (A) The current MIRCA system setup includes 2 FlowEZ pressure pumps with a Link module for automated pressure control, a control circuit that interfaces with 2 MIRCA chips for signal acquisition and processing, and a 7-inch touchscreen display. (B) Illustration of the MIRCA assay steps, in which isolated RBCs are either incubated in PBS (under NOI) or sodium metabisulfite (MBS) (under HOI) and perfused through a micropillar network in which poorly deformable RBCs occlude. (C) Brightfield images revealing the difference in occlusion levels for a typical HbSS sample under NOI and HOI in the 3 μm array. Arrows illustrate RBCs stuck in the micropillars with more RBCs in HOI compared with NOI. (D) Time course of occlusion indices obtained through the MIRCA device for a typical HbSS sample in NOI and HOI during sample perfusion.